Disentangling invasion processes in a dynamic shipping–boating network
Molecular Ecology (2012) 21, 4227–4241
ANAI¨S LACOURSIE` RE-ROUSSEL,* DAN G. BOCK,† MELANIA E. CRISTESCU,† FRE ´ DE´ RIC GUICHARD,* PHILIPPE GIRARD,‡ PIERRE LEGENDRE§ and CHRISTOPHER W. MCKINDSEY–
*Department of Biology, McGill University, 1205 Docteur Penfield, Montre´al, Que´bec, Canada H3A 1B1, †Great Lakes Institute
for Environmental Research, University of Windsor, Windsor, Ontario, Canada N9B 3P4, ‡De´partement de Ge´ographie,
Universite´ de Montre´al, C.P. 6128, Succursale Centre-Ville, Montre´al, Que´bec, Canada H3C 3J7, §De´partement de Sciences
Biologiques, Universite´ de Montre´al, C.P. 6128, Succursale Centre-Ville, Montre´al, Que´bec, Canada H3C 3J7, –Coastal and
Benthic Ecology, Institut Maurice-Lamontagne, Fisheries and Oceans Canada, C.P. 1000 Mont-Joli, Que´bec, Canada G5H 3Z4
Abstract
The relative importance of multiple vectors to the initial establishment, spread and population dynamics of invasive species remains poorly understood. This study used molecular methods to clarify the roles of commercial shipping and recreational boating in the invasion by the cosmopolitan tunicate, Botryllus schlosseri. We evaluated (i) single vs. multiple introduction scenarios, (ii) the relative importance of shipping and boating to primary introductions, (iii) the interaction between these vectors for spread (i.e. the presence of a shipping-boating network) and (iv) the role of boating in determining population similarity. Tunicates were sampled from 26 populations along the Nova Scotia, Canada, coast that were exposed to either shipping (i.e. ports) or boating (i.e. marinas) activities.Atotal of 874 individuals (c. 30 per population)fromfive portsand21 marinaswascollectedandanalysedusingbothmitochondrialcytochromecoxidase subunit I gene (COI) and 10 nuclear microsatellite markers. The geographical location of multiple hotspot populations indicates that multiple invasions have occurred in Nova Scotia. A loss of genetic diversity from port to marina populations suggests a stronger influence of ships than recreational boats on primary coastal introductions. Population genetic similarity analysis reveals a dependence of marina populations on those that had been previously established in ports. Empirical data on marina connectivity because of boating better explains patterns in population similarities than does natural spread. We conclude that frequent primary introductions arise by ships and that secondary spread occurs gradually thereafter around individual ports, facilitated by recreational boating.
Keywords: population connectivity, population dynamics, primary introduction, spread, tunicate Received 10 February 2012; revision received 9 May 2012; accepted 25 May 2012