Charlie
Well-Known Member
From: Fred Kibenge
To: Simon Jones@dfo-mpo.gc.ca
Date: 14/11/201111:28 AM
Subject: RE: Re: Paper
CC: Laura Richards; Mark Higgins; Mark Saunders; Molly Kibenge; Stewart ...
Dear Simon,
Thank you for your input on the publication question and for the information about the Cohen Commission.
For your information, this data (with your qualifiers below) is being relayed to CFIA as part of their on-going investigation. Our lab is currently making preparations for participation in this process and will disclose this work notwithstanding its age. I think that this historical data may also clarify some of the issues around recent ISAV testing in BC.
As you likely know, the OlE Manual of Diagnostic Tests for Aquatic Animals 2010 (http://www.cabi.org/ac/default.aspx?site= 162&page=332S) in the chapter on ISAV (http://www.oie.int/fileadmin/Home/eng/Health_standards/aahm/2010/2.3.0S_ISA .pdf) lists both criteria for suspicion and for confirmation of ISAV infection. This manuscript, which describes a targeted surveillance with significant prevalence rates of suspected infection in specific Pacific salmon species, accompanied by virus sequence data, may assist the CFlA.
Thanks.
Fred.
»> "Jones, Simon" <SimonJones@dfo-mpo.gc.ca> 11/4/2011 8:35 PM »>
Dear Molly,
Thanks for the message and for asking permission to submit the draft manuscript for publication.
Your email is timely. Recent events in BC concerning the alleged detection of ISA virus in wild Pacific salmon brought to mind the research you conducted while you were an NSERC Visiting Fellow at the Pacific Biological Station, and some of the questions it raised.
You may recall that Fish Health staff at DFO disagreed that your data supported the conclusion that ISA virus, whether asymptomatic or otherwise, occurred in the salmon you examined. For example, all attempts to isolate the virus into cell culture failed. As you are aware, the OlE case definition for ISAV infection requires either the isolation and identification of the virus into cell culture from two independent samples taken on two separate occasions, or the isolation and identification of ISAV in cell culture plus RT-PCR or serologic confirmation from tissue preparations. You may further recall that an independent laboratory was unable, on more than one occasion, to reproduce your RT-PCR results on the same samples. In my opinion, it will be very important to better understand the disagreement in laboratory results and to better test the hypothesis of "Asymptomatic ISAV" before moving towards publication.
On the related issue, CFIA is now conducting confirmatory testing of more recent samples from Pacific salmon in which initial positive RT-PCR results for ISAV were obtained. In addition, the Cohen Commission will reconvene for two days in December to hear evidence on ISA virus in British Columbia. I will wait to hear the outcome of these processes before further discussion on a 7-year-old manuscript. Consequently, I do not give permission to submit this work, whether in this manuscript or any other, for publication.
With my best wishes,
Simon
Dr. Simon R.M. Jones
Aquatic Animal Health Section
Pacific Biological Station
Fisheries and Oceans Canada
3190 Hammond Bay Road
Nanaimo, British Columbia
V9T 6N7, Canada
Tel: 2507298351
Fax: 250756 7053
E-mail: simon.jones@dfo-mpo.gc.ca
From: Molly Kibenge [mailto:Mkibenge@upeLca]
Sent: November 4,2011 7:19 AM
To: Jones, Simon
Cc: Fred Kibenge
Subject: Fwd: Re: Paper
Dear Simon,
I hope all is well in Nanaimo. It has been long since we last corresponded.
I am writing to follow up on the work I did in your lab during my post-doc at DFO-PBS, Nanaimo. As you recall we prepared a draft manuscript (attached). I am writing to ask your permission to submit it for publication as soon as possible. I would like to submit it to Diseases of Aquatic Organisms or Journal of Fish Diseases. What do you think?
Please let me know. If you have additional changes, please send them along and I will then proceed from there.
Please say hello to the family.
Best regards,
Molly.
To: Simon Jones@dfo-mpo.gc.ca
Date: 14/11/201111:28 AM
Subject: RE: Re: Paper
CC: Laura Richards; Mark Higgins; Mark Saunders; Molly Kibenge; Stewart ...
Dear Simon,
Thank you for your input on the publication question and for the information about the Cohen Commission.
For your information, this data (with your qualifiers below) is being relayed to CFIA as part of their on-going investigation. Our lab is currently making preparations for participation in this process and will disclose this work notwithstanding its age. I think that this historical data may also clarify some of the issues around recent ISAV testing in BC.
As you likely know, the OlE Manual of Diagnostic Tests for Aquatic Animals 2010 (http://www.cabi.org/ac/default.aspx?site= 162&page=332S) in the chapter on ISAV (http://www.oie.int/fileadmin/Home/eng/Health_standards/aahm/2010/2.3.0S_ISA .pdf) lists both criteria for suspicion and for confirmation of ISAV infection. This manuscript, which describes a targeted surveillance with significant prevalence rates of suspected infection in specific Pacific salmon species, accompanied by virus sequence data, may assist the CFlA.
Thanks.
Fred.
»> "Jones, Simon" <SimonJones@dfo-mpo.gc.ca> 11/4/2011 8:35 PM »>
Dear Molly,
Thanks for the message and for asking permission to submit the draft manuscript for publication.
Your email is timely. Recent events in BC concerning the alleged detection of ISA virus in wild Pacific salmon brought to mind the research you conducted while you were an NSERC Visiting Fellow at the Pacific Biological Station, and some of the questions it raised.
You may recall that Fish Health staff at DFO disagreed that your data supported the conclusion that ISA virus, whether asymptomatic or otherwise, occurred in the salmon you examined. For example, all attempts to isolate the virus into cell culture failed. As you are aware, the OlE case definition for ISAV infection requires either the isolation and identification of the virus into cell culture from two independent samples taken on two separate occasions, or the isolation and identification of ISAV in cell culture plus RT-PCR or serologic confirmation from tissue preparations. You may further recall that an independent laboratory was unable, on more than one occasion, to reproduce your RT-PCR results on the same samples. In my opinion, it will be very important to better understand the disagreement in laboratory results and to better test the hypothesis of "Asymptomatic ISAV" before moving towards publication.
On the related issue, CFIA is now conducting confirmatory testing of more recent samples from Pacific salmon in which initial positive RT-PCR results for ISAV were obtained. In addition, the Cohen Commission will reconvene for two days in December to hear evidence on ISA virus in British Columbia. I will wait to hear the outcome of these processes before further discussion on a 7-year-old manuscript. Consequently, I do not give permission to submit this work, whether in this manuscript or any other, for publication.
With my best wishes,
Simon
Dr. Simon R.M. Jones
Aquatic Animal Health Section
Pacific Biological Station
Fisheries and Oceans Canada
3190 Hammond Bay Road
Nanaimo, British Columbia
V9T 6N7, Canada
Tel: 2507298351
Fax: 250756 7053
E-mail: simon.jones@dfo-mpo.gc.ca
From: Molly Kibenge [mailto:Mkibenge@upeLca]
Sent: November 4,2011 7:19 AM
To: Jones, Simon
Cc: Fred Kibenge
Subject: Fwd: Re: Paper
Dear Simon,
I hope all is well in Nanaimo. It has been long since we last corresponded.
I am writing to follow up on the work I did in your lab during my post-doc at DFO-PBS, Nanaimo. As you recall we prepared a draft manuscript (attached). I am writing to ask your permission to submit it for publication as soon as possible. I would like to submit it to Diseases of Aquatic Organisms or Journal of Fish Diseases. What do you think?
Please let me know. If you have additional changes, please send them along and I will then proceed from there.
Please say hello to the family.
Best regards,
Molly.